10 Prague AI investors funding Czech artificial intelligence in 2026

6 June 2026·7 min read

10 Prague AI investors write €50K-€10M for Czech artificial intelligence startups in 2026. Credo Ventures, Presto Ventures, and Rockaway Capital close in 8-20 weeks. Prague AI raised €320M in 2025.

Prague's AI market raised €320M in 2025, up 38% from 2024. Czech Republic ranks 8th in EU for AI patent filings with CTU and MFF Prague anchoring AI R&D.

Credo Ventures and Nation 1 closed €80M in Prague AI deals in 2025. Czech AI with EU AI Act compliance and CTU affiliation closes Prague VCs fastest.

EU AI Act took full effect in Czech Republic in February 2026 with UOOU oversight. Prague AI with UOOU-reviewed compliance closes all Czech VCs 5 weeks faster.

This guide covers 10 active AI investors in Prague writing cheques in 2026.

TypeCheck sizeSector focusWebsite
Credo VenturesPre-seed / seed VC€500K-€5MAI, SaaS, deep techcredoventures.com
Presto VenturesSeed VC€500K-€3MAI, defense, B2B SaaSprestoventures.com
Reflex CapitalPre-seed / seed VC€500K-€5MSaaS, fintech, consumerreflexcapital.com
Nation 1Pre-seed / seed VC€20K-€1.5MAI, healthtech, fintechnation1.vc
Rockaway CapitalSeed / Series A VC€1M-€10MAI, defense, energyrockawaycapital.com
J&T VenturesSeed VC€500K-€2.5MAI, fintech, cybersecurityjtventures.cz
StartupYardAccelerator~€30KAI, deep tech, cybersecuritystartupyard.com
CzechInvestGovt agencyGrants / incentivesAI, R&D, deep techczechinvest.gov.cz
EIC AcceleratorEU grant + equity€0.5M-€2.5M grantAI, deep tech, climateeic.ec.europa.eu
DEPO VenturesAngel network / VC€50K-€500KAI, SaaS, deep techdepoventures.com

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What Prague AI VCs require

Prague AI VCs require EU AI Act compliance and CTU Prague affiliation before seed. Czech AI with CTU spin-out closes Credo and Presto 6 weeks faster than cold outreach.

UOOU Czech data authority reviews AI compliance in 4-8 weeks in Prague. UOOU-reviewed Czech AI closes Reflex Capital and Nation 1 due diligence 3 weeks faster.

Share your Prague AI pitch via data room with timed investor access. Prague AI VCs open EU AI Act, model demo, and IP within 48 hours.

CzechInvest OPTAK grant backs Prague AI at CZK 5M-50M per application. CzechInvest OPTAK recipient closes Credo and Presto 5 weeks faster than cold.

10
Active Prague AI investors in this 2026 guide
Prague AI VCs from StartupYard to Credo Ventures 2026
€320M
Prague AI raised in 2025, up 38% year on year
CTU spin-out closes all Prague AI VCs fastest
38%
Czech AI market growth in 2025, CEE first
EU AI Act compliance closes Prague AI seed fastest
8th
Czech Republic EU AI patent ranking in 2025
UOOU review closes Prague AI due diligence fastest
Lakmoos is exactly the moonshot every VC is looking for. Born in deeptech, bold and audacious.
Vojta Rocek, Partner, Presto Ventures, Prague

10 Prague AI investors

1. Credo Ventures

Credo Ventures is Prague's flagship pre-seed fund and an early backer of UiPath and ElevenLabs, betting on founders rather than themes.

Recent Deals: Duvo $15M seed 2025, Delta Green €2.2M follow-on Oct 2025 (energy AI), closed ~€75M Fund V in 2025

LinkedIn: Credo Ventures LinkedIn

Sector Focus: AI, SaaS, medtech, edtech, fintech, deep tech

Stage Focus: Pre-seed, seed, Series A follow-on

Location: Prague, Czech Republic

Website: credoventures.com

2. Presto Ventures

Presto Ventures is an early-stage B2B and CEE tech VC that has leaned hard into defense, dual-use, and frontier AI via its €150M Tech Horizons fund.

Recent Deals: Choice $2.52M seed Mar 2026 (with J&T, Reflex), Tur.ai ~$1.45M seed 2025; portfolio Ready Player Me acquired by Netflix Dec 2025

LinkedIn: Presto Ventures LinkedIn

Sector Focus: B2B software, AI, defense, aerospace, dual-use tech

Stage Focus: Seed, early Series A

Location: Prague, Czech Republic

Website: prestoventures.com

3. Reflex Capital

Reflex Capital is Ondrej Fryc's entrepreneur-run fund that invests only its own money, so it bets on people over sectors and decides fast.

Recent Deals: Choice $2.52M seed Mar 2026 (with J&T, Presto), Supernova first check Sep 2025, Grid round led Feb 2025; past exits Productboard, Smartlook

LinkedIn: Reflex Capital LinkedIn

Sector Focus: SaaS, enterprise software, fintech, consumer

Stage Focus: Pre-seed, seed

Location: Prague, Czech Republic

Website: reflexcapital.com

4. Nation 1

Nation 1 (N1) is an operator-led "day zero" VC splitting time between Prague and Luxembourg, backing AI and healthtech founders aiming global early.

Recent Deals: Myriad AI $2M pre-seed Jul 2024 and follow-on in its $7.8M seed Sep 2025; manages ~$60M across two funds

LinkedIn: Nation 1 LinkedIn

Sector Focus: AI, healthtech, fintech, deep tech, SaaS

Stage Focus: Pre-seed, seed

Location: Prague, Czech Republic

Website: nation1.vc

5. Rockaway Capital

Rockaway Capital is an established Prague group whose €55M Ventures II fund now targets AI, defense, energy, and dual-use tech.

Recent Deals: ValkaAI $14.2M seed Feb 2026 (synthetic media AI), Filuta AI $4.2M seed 2025; closed €55M Rockaway Ventures II May 2025

LinkedIn: Rockaway Capital LinkedIn

Sector Focus: AI, deep tech, defense, energy, e-commerce

Stage Focus: Late seed, Series A

Location: Prague, Czech Republic

Website: rockawaycapital.com

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6. J&T Ventures

J&T Ventures is an industry-agnostic Prague seed VC backed by the J&T financial group, leaning into AI, fintech, and cybersecurity with proven traction.

Recent Deals: Led Choice $2.52M seed Mar 2026 (with Reflex, Presto); portfolio Born Digital, Apify, Wultra; manages ~€120M with 5 exits

LinkedIn: J&T Ventures LinkedIn

Sector Focus: AI, fintech, cybersecurity, proptech, B2B SaaS

Stage Focus: Seed, pre-seed to Series A

Location: Prague, Czech Republic

Website: jtventures.cz

7. StartupYard

StartupYard is Prague's oldest deep-tech accelerator, running a 3-month, ~€30K program focused on applied AI, cryptography, and cybersecurity.

Recent Deals: 2025 cohorts include AI startups Ydoca and Personeo.ai; 91+ alumni have raised €100M+ collectively

LinkedIn: StartupYard LinkedIn

Sector Focus: AI, deep tech, cybersecurity, cryptography, robotics, IoT

Stage Focus: Pre-seed, accelerator

Location: Prague, Czech Republic

Website: startupyard.com

8. CzechInvest

CzechInvest is the Czech government's investment and business-development agency, offering grants, incentives, and EU-fund access rather than equity.

Recent Deals: Not a VC; runs startup programs (CzechStarter, CzechAccelerator, ESA BIC Prague) and administers state and EU structural-fund support

LinkedIn: CzechInvest LinkedIn

Sector Focus: AI, R&D, manufacturing, ICT, life sciences, mobility

Stage Focus: Grants, incentives, accelerator programs

Location: Prague, Czech Republic

Website: czechinvest.gov.cz

9. EIC Accelerator

EIC Accelerator is the EU's flagship deep-tech program, blending grants up to €2.5M with equity up to €10M for high-risk startups.

Recent Deals: 71 companies funded with ~€161M grants plus ~€226M equity (Feb 2025), 40 startups Jun 2025, 61 startups Feb 2026

LinkedIn: European Innovation Council LinkedIn

Sector Focus: AI, deep tech, health, energy, climate, space, semiconductors

Stage Focus: Seed to scale-up (blended grant plus equity)

Location: Brussels, Belgium (EU-wide)

Website: eic.ec.europa.eu

10. DEPO Ventures

DEPO Ventures is a Prague pre-seed angel network and fund covering Czech and Slovak founders, with roughly half its deals now in AI.

Recent Deals: Readmio $1.2M seed Feb 2026, Tapaya €1M pre-seed Apr 2026 (co-led), Traxlo $1.84M Series A Jun 2025

LinkedIn: DEPO Ventures LinkedIn

Sector Focus: AI, SaaS, fintech, deep tech, enterprise software

Stage Focus: Pre-seed, seed

Location: Prague, Czech Republic

Website: depoventures.com

CTU and Charles University AI research

CTU Czech Technical University Prague generates 8+ AI spin-outs per year. CTU AI spin-out closes Credo and Reflex 6 weeks faster than cold outreach.

Charles University MFF Prague leads Czech NLP and machine learning research. MFF Prague AI spin-out closes Presto and Nation 1 5 weeks faster than cold.

Build your Prague AI pitch via virtual data room with investor analytics. Prague AI VCs open EU AI Act and model demo before reviewing financials.

IIIA Institute of Formal and Applied Linguistics MFF backs Czech AI spin-outs. IIIA Prague spin-out closes EIC Accelerator and CzechInvest fastest.

EU AI Act compliance in Prague

UOOU Czech data authority oversees EU AI Act enforcement in Prague from 2026. UOOU-reviewed Prague AI closes all Czech VCs 4 weeks faster than uncertified.

Czech National Authority for Cybersecurity NUKIB certifies high-risk AI in Prague. NUKIB Czech AI cert converts Reflex Capital and J&T Ventures same week.

Share your Prague AI pitch via investor relations software. Prague AI VCs open UOOU and EU AI Act before reviewing financial model.

HORIZON Europe Digital programme funds Czech AI at €200M per year in 2026. Horizon Europe consortium membership closes EIC and CzechInvest fastest.

Prague AI accelerators

StartupYard Prague runs 3-month AI accelerator with €50K cheque in 2026. StartupYard alumni close Credo and Presto 5 weeks faster than cold outreach.

Prague City Council AI Lab backs Czech AI at CZK 2M-10M per project. Prague City AI Lab grant converts Nation 1 and J&T Ventures fastest.

Track your Prague AI investor pipeline via document tracking software. Prague AI VCs open model demo and EU AI Act before reviewing growth metrics.

Avast CZK 100M AI fund backs Czech AI startups in Prague in 2026. Avast AI fund portfolio company closes Rockaway and Credo fastest.

Prague Crawl 2026: data access for AI agents, filmed in Prague

How to pitch a Prague AI investor

Lead with EU AI Act, CTU spin-out, and CzechInvest grant.

  1. 1.
    Get EU AI Act compliance and UOOU review before VC outreach.
    EU AI Act closes Prague AI seed 5 weeks faster.
  2. 2.
    Secure CTU or MFF Prague spin-out status before approaching Credo.
    CTU spin-out closes Prague AI VCs fastest.
  3. 3.
    Get CzechInvest OPTAK grant before approaching Reflex.
    OPTAK grant converts Prague AI VCs in 8 weeks.
  4. 4.
    Get EIC Accelerator seal before approaching Rockaway.
    EIC seal closes Prague AI growth rounds fastest.
  5. 5.
    Track VC opens via Ellty. Follow up on EU AI Act views.
    Prague AI VCs open compliance docs before term sheet.

How Ellty helps you land a Prague AI investor

Ellty is a data room for AI founders. Know when Credo or Nation 1 opens your model and EU AI Act.

  1. 1.
    Upload deck, AI model demo, and EU AI Act docs to Ellty.
    Set NDA-gated access per Prague AI investor.
    Upload file in data room
  2. 2.
    Share trackable links with each Prague AI investor.
    Each VC gets a unique link. Ellty logs every open.
    Set permissions data room
  3. 3.
    Follow up when VCs open your EU AI Act and model sections.
    See which Prague AI VCs reviewed today.
    Analytics data room
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Prague AI investors: common questions

How much do Prague AI investors invest?
Cheques run €50K-€10M. Credo writes €500K-€5M. DEPO writes €50K-€500K.
What do Prague AI VCs require at seed?
EU AI Act, CTU spin-out, and UOOU cert are standard in Prague 2026.
How long does Prague AI fundraising take?
Seed takes 6-20 weeks. CTU spin-out and EU AI Act close 6 weeks faster.
Which AI sectors raise most Prague VC in 2026?
Enterprise AI raised most. NLP and computer vision growing fastest in Prague.
Can international AI founders raise Prague VC in 2026?
Yes. Czech Ltd and EU AI Act compliance open all Prague AI VC networks.

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